CDC16 antibody (N-Term)

Catalog No. ABIN6991326

This anti-CDC16 antibody is a Rabbit Polyclonal antibody detecting CDC16 in WB, ELISA, IF and IHC (p). Suitable for Human and Mouse.

Quick Overview for CDC16 antibody (N-Term) (ABIN6991326)

Target: CDC16 (Cell Division Cycle 16 Homolog (S. Cerevisiae) (CDC16))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC16 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CDC16 Antibody

Binding Specificity: N-Term

Purification: APC6 Antibody is affinity chromatography purified via peptide column.

Immunogen: APC6 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human APC6. The immunogen is located within the first 50 amino acids of APC6.

Isotype: IgG

Application Details

Application Notes: APC6 antibody can be used for detection of APC6 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: APC6 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: APC6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for CDC16

Target: CDC16 (Cell Division Cycle 16 Homolog (S. Cerevisiae) (CDC16))

Alternative Name: APC6

Background: APC6 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC6 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. While little is known of APC6, it has been shown to interact with and is stabilized by CDC26 through an intermolecular TPR mimic composed of one helix from each protein.

Gene ID: 8881

NCBI Accession: NP_003894

UniProt: Q13042

Pathways: Regulation of Actin Filament Polymerization