CDC23 antibody (AA 270-320)

Catalog No. ABIN6991328

The Rabbit Polyclonal anti-CDC23 antibody has been validated for WB, ELISA and ICC. It is suitable to detect CDC23 in samples from Human, Mouse and Rat.

Quick Overview for CDC23 antibody (AA 270-320) (ABIN6991328)

Target: CDC23 (Cell Division Cycle 23 (CDC23))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC23 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Product Details anti-CDC23 Antibody

Binding Specificity: AA 270-320

Purification: APC8 Antibody is affinity chromatography purified via peptide column.

Immunogen: APC8 antibody was raised against a 17 amino acid synthetic peptide near the center of human APC8. The immunogen is located within amino acids 270 - 320 of APC8.

Isotype: IgG

Application Details

Application Notes: APC8 antibody can be used for detection of APC8 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 5 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: APC8 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: APC8 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for CDC23

Target: CDC23 (Cell Division Cycle 23 (CDC23))

Alternative Name: APC8

Background: APC8 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC8 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. In Drosophila, silencing of APC8 results in developmental delay and pupal lethality with elevated levels of apoptosis, high mitotic index, and delayed or blocked mitosis.

Gene ID: 8697

UniProt: Q9UJX2

Pathways: Protein targeting to Nucleus