ANAPC10 antibody (AA 60-110)

Catalog No. ABIN6991329

This anti-ANAPC10 antibody is a Rabbit Polyclonal antibody detecting ANAPC10 in WB, ELISA, IF and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for ANAPC10 antibody (AA 60-110) (ABIN6991329)

Target: ANAPC10 (Anaphase Promoting Complex Subunit 10 (ANAPC10))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ANAPC10 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ANAPC10 Antibody

Binding Specificity: AA 60-110

Purification: APC10 Antibody is affinity chromatography purified via peptide column.

Immunogen: APC10 antibody was raised against a 16 amino acid synthetic peptide near the center of human APC10. The immunogen is located within amino acids 60 - 110 of APC10.

Isotype: IgG

Application Details

Application Notes: APC10 antibody can be used for detection of APC10 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: APC10 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: APC10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for ANAPC10

Target: ANAPC10 (Anaphase Promoting Complex Subunit 10 (ANAPC10))

Alternative Name: APC10

Background: APC10 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC10 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. It has been suggested that APC10 plays a role to regulate the binding of specific substrates to the APC/C complex, similar to that of coactivators.

Gene ID: 10393

UniProt: Q9UM13

Pathways: M Phase