CXXC4 antibody (N-Term)

Catalog No. ABIN6991438

This Rabbit Polyclonal antibody specifically detects CXXC4 in WB, ELISA, IHC (p) and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CXXC4 antibody (N-Term) (ABIN6991438)

Target: CXXC4 (CXXC Finger Protein 4 (CXXC4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXXC4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-CXXC4 Antibody

Binding Specificity: AA 50-100, N-Term

Purification: CXXC4 Antibody is affinity chromatography purified via peptide column.

Immunogen: CXXC4 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human CXXC4. The immunogen is located within amino acids 50 - 100 of CXXC4.

Isotype: IgG

Application Details

Application Notes: CXXC4 antibody can be used for detection of CXXC4 by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 10 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: CXXC4 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: CXXC4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for CXXC4

Target: CXXC4 (CXXC Finger Protein 4 (CXXC4))

Alternative Name: CXXC4

Background: CXXC4 Antibody: CXXC4 is zinc finger protein that binds directly to the PDZ domain of DVL1, a protein involved in the Wnt signaling pathway, through its C-terminal region. CXXC4's interaction with DVL1 competes with DVL1-AXIN binding, although the affinity of DVL1 for CXXC4 is lower than that for AXIN. In mouse fibroblasts, CXXC4 suppressed Wnt3a induction of beta-catenin and inhibited Wnt3a-dependent TCF4 activation, suggesting that CXXC4 acts as a negative regulator of the Wnt signaling pathway and functions between DVL and beta-catenin. Recent reports suggest that decreased CXXC4 expression is associated with kidney cancer metastasis, cell proliferation, reduced apoptosis in response to cancer drugs, and upregulation of genes involved with proliferation, invasion and cell survival, suggesting that CXXC4 plays a critical role in tumor progression in kidney cancer.

Gene ID: 80319

NCBI Accession: NP_079488

UniProt: Q9H2H0