The Rabbit Polyclonal anti-DPF2 antibody is suitable to detect DPF2 in samples from Human and Mouse. It has been validated for WB, ELISA, IF and IHC (p).
Requiem antibody is predicted to not cross-react with other DPF protein family members.
Purification
REQUIEM Antibody is affinity chromatography purified via peptide column.
Immunogen
REQUIEM antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human Requiem. The immunogen is located within the last 50 amino acids of REQUIEM.
Verified
DPF2
Reactivity: Human
WB, ELISA
Host: Goat
Polyclonal
unconjugated
Application Notes
REQUIEM antibody can be used for detection of REQUIEM by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in mouse samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
REQUIEM Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
REQUIEM antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
DPF2
(Zinc finger protein ubi-d4 (DPF2))
Alternative Name
REQUIEM
Background
REQUIEM Antibody: Requiem is a member of the d4 domain family, characterized by a zinc finger-like structural motif. This protein functions as a transcription factor which is necessary for the apoptotic response following deprivation of survival factors. It is expressed in numerous tissues in both cytosolic and nuclear regions and likely serves a regulatory role in rapid hematopoietic cell growth and turnover. Requiem has been found to act as an adaptor protein that links the NF-κ,B and SWI/SNF chromatin remodeling factor and is considered a candidate gene for multiple endocrine neoplasia type I, an inherited cancer syndrome involving multiple parathyroid, enteropancreatic, and pituitary tumors.