ZBTB5 antibody (C-Term)

Catalog No. ABIN6991469

The Rabbit Polyclonal anti-ZBTB5 antibody has been validated for WB, ELISA, IHC (p) and IF. It is suitable to detect ZBTB5 in samples from Human, Mouse and Rat.

Quick Overview for ZBTB5 antibody (C-Term) (ABIN6991469)

Target: ZBTB5 (Zinc Finger and BTB Domain Containing 5 (ZBTB5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZBTB5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-ZBTB5 Antibody

Binding Specificity: C-Term

Specificity: At least two isoforms of ZBTB5 are known to exist, this antibody will recognize both isoforms. This antibody is predicted to not cross-react with other ZBTB protein family members.

Purification: ZBTB5 Antibody is affinity chromatography purified via peptide column.

Immunogen: ZBTB5 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human ZBTB6. The immunogen is located within the last 50 amino acids of ZBTB5.

Isotype: IgG

Application Details

Application Notes: ZTBT5 antibody can be used for detection of ZTBT5 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: ZBTB5 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: ZBTB5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for ZBTB5

Target: ZBTB5 (Zinc Finger and BTB Domain Containing 5 (ZBTB5))

Alternative Name: ZBTB5

Background: ZBTB5 Antibody: The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. ZBTB5 was identified though sequence analysis as a POZ domain Kruppel-like zinc finger (POK) protein. Further experiments indicated that it binds DNA and can directly repress transcription of the cell cycle arrest gene p21. ZBTB5 can also interact with co-repressor histone deacetylase complexes such as BCoR, NCoR, and SMRT via its POZ domain, resulting in deacetylation of histones Ac-H3 and Ac-H4 at the proximal promoter. ZBTB5 stimulates both cell proliferation and cell cycle progression, suggesting that it may act as a potential proto-oncogene.

Gene ID: 9925

NCBI Accession: NP_055687

UniProt: O15062