MSI2 antibody (N-Term)

Catalog No. ABIN6991532

The Rabbit Polyclonal anti-MSI2 antibody has been validated for WB, ELISA, IF, IHC (p) and ICC. It is suitable to detect MSI2 in samples from Human, Mouse and Rat.

Quick Overview for MSI2 antibody (N-Term) (ABIN6991532)

Target: MSI2 (Musashi Homolog 2 (MSI2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MSI2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-MSI2 Antibody

Binding Specificity: AA 30-80, N-Term

Specificity: MSI2 antibody is predicted to not cross-react with MSI1. Multiple isoforms of MSI2 are known to exist.

Purification: MSI2 Antibody is affinity chromatography purified via peptide column.

Immunogen: MSI2 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human MSI2. The immunogen is located within amino acids 30 - 80 of MSI2.

Isotype: IgG

Application Details

Application Notes: MSI2 antibody can be used for detection of MSI2 by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL and immunocytochemistry starting at 5 μ,g/mL. For immunofluorescence start at 5 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in mouse samples, Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: MSI2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: MSI2 antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for MSI2

Target: MSI2 (Musashi Homolog 2 (MSI2))

Alternative Name: MSI2

Background: MSI2 Antibody: Musashi2 (MSI2) is an RNA-binding protein that is highly expressed in precursor cells in the ventricular and subventricular zones of the developing mammalian CNS. Like the related MSI1, MSI2 has been suggested to be involved stem cell production and maintenance. MSI2 is the predominant MSI protein in hematopoietic stem cells, and its knockdown leads to reduced engraftment and depletion in vivo. Expression levels of MSI2 are elevated in myeloid leukemia cells lines, and MSI2 appears to cooperate with BCR-ABL1 to induce an aggressive leukemia, the level of MSI2 directly correlates with decreased survival in patients. MSI2 negatively regulates the asymmetric cell fate determinant NUMB, suggesting that this signaling pathway may provide future targets for future therapies.

Gene ID: 124540

UniProt: Q96DH6