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SPATA1 antibody (C-Term)

This Rabbit Polyclonal antibody specifically detects SPATA1 in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Human and Mouse.
Catalog No. ABIN6991600

Quick Overview for SPATA1 antibody (C-Term) (ABIN6991600)

Target

SPATA1 (Spermatogenesis Associated 1 (SPATA1))

Reactivity

Human, Mouse

Host

  • 1
Rabbit

Clonality

  • 1
Polyclonal

Conjugate

  • 1
This SPATA1 antibody is un-conjugated

Application

Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Binding Specificity

    AA 300-350, C-Term

    Specificity

    At least two isoforms of SPATA1 are known to exist, this antibody will detect only the longest isoforms. SPATA1 antibody is predicted to not cross-react with other SPATA family members.

    Purification

    SPATA1 Antibody is affinity chromatography purified via peptide column.

    Immunogen

    SPATA1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SPATA1. The immunogen is located within amino acids 300 - 350 of SPATA1.

    Isotype

    IgG
  • Application Notes

    SPATA1 antibody can be used for detection of SPATA1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

    Antibody validated: Western Blot in mouse samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    SPATA1 Antibody is supplied in PBS containing 0.02 % sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C,4 °C

    Storage Comment

    SPATA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
  • Target

    SPATA1 (Spermatogenesis Associated 1 (SPATA1))

    Alternative Name

    SPATA1

    Background

    SPATA1 Antibody: SPATA1, a novel cell-surface protein, is involved in shaping the sperm head during spermatogenesis. SPATA1 is localized at chromosome 1q21. At least three isoforms of SPATA1 are known to exist. The longest is preferentially expressed in hematopoietic tissues. Its extracellular domain contains a single immunoglobulin-like domain, and its intracellular segment has two immunoreceptor tyrosine-based inhibition motifs (ITIMs). It is thought to have an important role in hematopoietic cell signaling. The mid-sized isoform contains a short intracellular part without ITIMs, while the shortest isoform lacks the transmembrane segment and represents a potential soluble protein (2, 3).

    Gene ID

    100505741

    UniProt

    Q5VX52
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