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SPATA3 antibody (C-Term)

The Rabbit Polyclonal anti-SPATA3 antibody has been validated for ELISA, WB, IF and IHC (p). It is suitable to detect SPATA3 in samples from Human and Mouse.
Catalog No. ABIN6991601

Quick Overview for SPATA3 antibody (C-Term) (ABIN6991601)

Target

SPATA3 (Spermatogenesis Associated 3 (SPATA3))

Reactivity

Human, Mouse

Host

  • 1
Rabbit

Clonality

  • 1
Polyclonal

Conjugate

  • 1
This SPATA3 antibody is un-conjugated

Application

  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Binding Specificity

    C-Term

    Specificity

    At least three isoforms of SPATA3 are known to exist, this antibody will detect only the longest two isoforms. SPATA3 antibody is predicted to not cross-react with SPATA protein family members

    Purification

    SPATA3 Antibody is affinity chromatography purified via peptide column.

    Immunogen

    SPATA3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SPATA3. The immunogen is located within the last 50 amino acids of SPATA3.

    Isotype

    IgG
  • Application Notes

    SPATA3 antibody can be used for detection of SPATA3 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

    Antibody validated: Western Blot in mouse samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    SPATA3 Antibody is supplied in PBS containing 0.02 % sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C,4 °C

    Storage Comment

    SPATA3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
  • Target

    SPATA3 (Spermatogenesis Associated 3 (SPATA3))

    Alternative Name

    SPATA3

    Background

    SPATA3 Antibody: The testis and spermatogenesis cell apoptosis related gene 1 (TSARG1) protein, also known as SPATA3, is highly expressed in testis, and to a lower extent in other tissues. SPATA3 contains a highly conserved J domain, which is present in all DnaJ-like proteins, and is thus a novel member of the HSP40 protein family. SPATA3 is thought to be involved in apoptosis of spermatogenic cells.

    Molecular Weight

    Predicted: 21 kDa

    Observed: 26 kDa

    Gene ID

    130560

    NCBI Accession

    NP_620712

    UniProt

    Q8NHX4
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