AFAP antibody (N-Term)

Catalog No. ABIN6991604

The Rabbit Polyclonal anti-AFAP antibody has been validated for WB and ELISA. It is suitable to detect AFAP in samples from Human and Mouse.

Quick Overview for AFAP antibody (N-Term) (ABIN6991604)

Target: AFAP (AFAP1) (Actin Filament Associated Protein 1 (AFAP1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AFAP antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-AFAP Antibody

Binding Specificity: AA 30-80, N-Term

Specificity: Monomer and homomultimer of AFAP1 are known to exist, AFAP1 antibody is predicted to not cross-react with other AFAP family members.

Purification: AFAP1 Antibody is affinity chromatography purified via peptide column.

Immunogen: AFAP1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human AFAP1. The immunogen is located within amino acids 30 - 80 of AFAP1.

Isotype: IgG

Application Details

Application Notes: AFAP1 antibody can be used for detection of AFAP1 by Western blot at 1 - 2 μ,g/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: AFAP1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: AFAP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for AFAP

Target: AFAP (AFAP1) (Actin Filament Associated Protein 1 (AFAP1))

Alternative Name: AFAP1

Background: AFAP1 Antibody: The actin filament-associated protein AFAP1 (AFAP-110) is an actin cross-linking protein first identified as a substrate of the viral oncogene v-Src. AFAP1 has a fundamental role in actin cytoskeleton arrangement. It contains a carboxyterminal actin-binding domain that directly binds to F-actin and serves as an adaptor protein in the regulation of SRC and PKC signal transduction by several functional domains, including 2 pleckstrin homology (PH) domains, a Src homology 3-binding (SH3-binding) motif, and several SH2-binding motifs. It is overexpressed in prostate carcinoma and contributes to tumor growth by regulating cell-matrix adhesions and migration in the cancer cells. AFAP1 represent a possible therapeutic target for controlling tumorigenesis and metastasis.

Gene ID: 60312

NCBI Accession: NP_940997

UniProt: Q8N556