AFAP antibody (Middle Region)

Catalog No. ABIN2787272

The Rabbit Polyclonal anti-AFAP antibody is suitable to detect AFAP in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for AFAP antibody (Middle Region) (ABIN2787272)

Target: AFAP (AFAP1) (Actin Filament Associated Protein 1 (AFAP1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AFAP antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-AFAP Antibody

Binding Specificity: Middle Region

Purpose: AFAP1 Antibody - middle region

Sequence: QVKRKKSSKS EAKGTVSKVT GKKITKIISL GKKKPSTDEQ TSSAEEDVPT

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against AFAP1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human AFAP1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP57552-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for AFAP

Target: AFAP (AFAP1) (Actin Filament Associated Protein 1 (AFAP1))

Alternative Name: AFAP1

Background:

Background Information: The protein encoded by this gene is a Src binding partner. It may represent a potential modulator of actin filament integrity in response to cellular signals, and may function as an adaptor protein by linking Src family members and/or other signaling proteins to actin filaments. Multiple transcript variants encoding different isoforms have been found for this gene.

Gene Name: Actin filament associated protein 1

Alternative Symbols: AFAP, AFAP110, AFAP-110

Protein Name: Actin filament-associated protein 1

Molecular Weight: 810kDa

Gene ID: 60312

NCBI Accession: NP_940997

UniProt: Q8N556