PRICKLE1 antibody (C-Term)

Catalog No. ABIN6991606

This Rabbit Polyclonal antibody specifically detects PRICKLE1 in WB, ELISA and IF. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for PRICKLE1 antibody (C-Term) (ABIN6991606)

Target: PRICKLE1 (Prickle-Like Protein 1 (PRICKLE1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRICKLE1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-PRICKLE1 Antibody

Binding Specificity: C-Term

Specificity: Multiple isoforms of PRICKLE1 are known to exist. PRICKLE1 antibody is predicted to not cross-react with other PRICKLE protein family members.

Purification: PRICKLE1 Antibody is affinity chromatography purified via peptide column.

Immunogen: PRICKLE1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human PRICKLE1. The immunogen is located within the last 50 amino acids of PRICKLE1.

Isotype: IgG

Application Details

Application Notes: PRICKLE1 antibody can be used for detection of PRICKLE1 by Western blot at 1 μ,g/mL. Antibody can also be used for immunofluorescence starting at 20 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PRICKLE1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: PRICKLE1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for PRICKLE1

Target: PRICKLE1 (Prickle-Like Protein 1 (PRICKLE1))

Alternative Name: PRICKLE1

Background: PRICKLE1 Antibody: PRICKLE1, also known as RILP or EPM1B, is a Disheveled-associated protein that serves as a nuclear translocation receptor for REST/NRSF and REST4 (1, 2). It contains three N-terminal LIM domains and three C-terminal nuclear localization signals. It localizes to the cytoplasm, as well as to the nuclear membrane and expressed at higher levels in placenta. PRICKLE1 is a negative regulator of the Wnt / beta-catenin signaling pathway and is a putative tumor suppressor in human HCCs. Defects in the gene encoding PRICKLE1 are associated with autosomal recessive progressive myoclonic epilepsy.

Molecular Weight: Predicted: 46, 91 kDa

Observed: 52, 90 kDa

Gene ID: 144165

NCBI Accession: NP_694571

UniProt: Q96MT3

Pathways: WNT Signaling, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development, Protein targeting to Nucleus