RAPGEF3 antibody (N-Term)

Catalog No. ABIN6991625

The Rabbit Polyclonal anti-RAPGEF3 antibody is suitable to detect RAPGEF3 in samples from Human, Rat and Mouse. It has been validated for WB, ELISA, IF and IHC (p).

Quick Overview for RAPGEF3 antibody (N-Term) (ABIN6991625)

Target: RAPGEF3 (Rap Guanine Nucleotide Exchange Factor (GEF) 3 (RAPGEF3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAPGEF3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RAPGEF3 Antibody

Binding Specificity: AA 60-110, N-Term

Specificity: At least two isoforms of EPAC1 are known to exist, this antibody will detect both isoforms. EPAC1 antibody is predicted to not cross-react with EPAC2.

Purification: EPAC1 Antibody is affinity chromatography purified via peptide column.

Immunogen: EPAC1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human EPAC1. The immunogen is located within amino acids 60 - 110 of EPAC1.

Isotype: IgG

Application Details

Application Notes: EPAC1 antibody can be used for detection of EPAC1 by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in rat samples, Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: EPAC1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: EPAC1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for RAPGEF3

Target: RAPGEF3 (Rap Guanine Nucleotide Exchange Factor (GEF) 3 (RAPGEF3))

Alternative Name: EPAC1

Background: EPAC1 Antibody: EPAC1, also known as Rap guanine nuclear exchange factor 3 and cAMPGEF-I, is widely expressed but most prominently in brain, heart, kidney, pancreas, spleen, ovary, thyroid and spinal cord. EPAC1 is a cAMP-binding protein with intrinsic guanine nuclear exchange factor activity that couples cAMP production to the activation of Rap, a GTPase belonging to the Ras family. This activation of Rap influences numerous cellular processes such as integrin-mediated cell adhesion, vascular endothelial barrier formation, and cardiac myocyte gap junction formation. Recently, EPAC1 has been suggested to also be involved in the cAMP-dependent regulation of ion channel formation, intracellular Ca++ signalling, ion transporter activity, and exocytosis.

Gene ID: 10411

NCBI Accession: NP_001092001

UniProt: O95398

Pathways: cAMP Metabolic Process