NKX2-4 antibody is predicted to not cross-react with other NK2 homeobox family members. At least two isoforms of NKX2-4 are known to exist, this antibody will detect both isoforms.
Purification
NKX2-4 Antibody is affinity chromatography purified via peptide column.
Immunogen
NKX2-4 antibody was raised against an 18 amino acid synthetic peptide near the center of human NKX2-4. The immunogen is located within amino acids 220 - 270 of NKX2-4.
NKX2-4
Reactivity: Human
WB, ELISA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
NKX2-4 antibody can be used for detection of NKX2-4 by Western blot at 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in mouse samples, Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
NKX2-4 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
NKX2-4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
NK2 Homeobox 4 (NKX2-4)
Alternative Name
NKX2-4
Background
NKX2-4 Antibody: NKX2-4 (NK2 homeobox 4) is a member of a family of transcription factors that are involved in embryonic development and cell fate. NKX2-4 is closely related to NKX2-1 and NKX2-4 and is specifically localized to the ventral diencephalon. Recent experiments in Xenopus have suggested that the homeodomain protein XANF is necessary for the activation of NKX2-4, but does not directly regulate its expression.