HHATL antibody (N-Term)

Catalog No. ABIN6991671

Product Details anti-HHATL Antibody

Target: HHATL (Hedgehog Acyltransferase-Like (HHATL))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HHATL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Purification: HHATL Antibody is affinity chromatography purified via peptide column.

Immunogen: HHATL antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human HHATL. The immunogen is located within the first 50 amino acids of HHATL.

Isotype: IgG

Application Details

Application Notes: HHATL antibody can be used for detection of HHATL by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 2.5 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: HHATL Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: HHATL antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for HHATL

Target: HHATL (Hedgehog Acyltransferase-Like (HHATL))

Alternative Name: HHATL (HHATL Products)

Background: HHATL Antibody: The Protein-cysteine N-palmitoyltransferase HHAT-like protein (HHATL), also known at the mammalian glycerol uptake/transporter 1 (Gup1) is a homolog of the S. cerevisiae Gup1. It is an endoplasmic reticulum (ER) membrane protein that co-localizes with the mammalian hedgehog acyltransferse Skn and interferes with its N-terminal palmitoylation of Sonic hedgehog (SHH), suggesting that HHATL acts as a negative regulator for N-terminal palmitoylation of SHH. HHATL is highly expressed in normal skin, but becomes down-regulated in skin squamous cell carcinoma, suggesting it may play a role in its development.

Gene ID: 57467

NCBI Accession: NP_065758

UniProt: Q9HCP6

Pathways: Hedgehog Signaling