FCHO2 antibody (AA 340-390)

Catalog No. ABIN6991694

The Rabbit Polyclonal anti-FCHO2 antibody has been validated for WB, ELISA, IF and IHC (p). It is suitable to detect FCHO2 in samples from Human, Mouse and Rat.

Quick Overview for FCHO2 antibody (AA 340-390) (ABIN6991694)

Target: FCHO2 (FCH Domain Only 2 (FCHO2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FCHO2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FCHO2 Antibody

Binding Specificity: AA 340-390

Specificity: FCHO2 antibody is predicted to not cross-react with other FCHO protein family members. At least the isoforms of FCHO2 are known to exist, this antibody will detect only the two longest isoforms.

Purification: FCHO2 Antibody is affinity chromatography purified via peptide column.

Immunogen: FCHO2 antibody was raised against a 15 amino acid synthetic peptide near the center of human FCHO2. The immunogen is located within amino acids 340 - 390 of FCHO2.

Isotype: IgG

Application Details

Application Notes: FCHO2 antibody can be used for detection of FCHO2 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 2.5 μ,g/mL.

Antibody validated: Western Blot in rat samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: FCHO2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: FCHO2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for FCHO2

Target: FCHO2 (FCH Domain Only 2 (FCHO2))

Alternative Name: FCHO2

Background: FCHO2 Antibody: FCHO2 (FCH domain only 2) is a ubiquitously expressed member of the FCFBS superfamily characterized by FES-CIP4 homology (FCH) domain, an FBH domain, and an SH3 domain. Both FCHO2 and the related protein FCHO1 are mediators of clathrin-mediated endocytosis, acting to sculpt the initial bud site and recruit the clathrin machinery for clathrin-coated vesicle formation. FCHO2 binds to Eps15, an important adaptor protein in clathrin-mediated endocytosis.

Gene ID: 115548

NCBI Accession: NP_620137

UniProt: Q0JRZ9