VAMP7 antibody (N-Term)

Catalog No. ABIN6991714

This Rabbit Polyclonal antibody specifically detects VAMP7 in ELISA, WB and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for VAMP7 antibody (N-Term) (ABIN6991714)

Target: VAMP7 (Vesicle-Associated Membrane Protein 7 (VAMP7))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VAMP7 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-VAMP7 Antibody

Binding Specificity: AA 20-70, N-Term

Specificity: At least three isoforms of VAMP7 are known to exist, this antibody will detect the two longest isoforms. This antibody is predicted to not cross-react with other Ras-related proteins.

Purification: VAMP7 Antibody is affinity chromatography purified via peptide column.

Immunogen: VAMP7 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human VAMP7. The immunogen is located within amino acids 20 - 70 of VAMP7.

Isotype: IgG

Application Details

Application Notes: VAMP7 antibody can be used for detection of VAMP7 by Western blot at 0.5 - 1 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: VAMP7 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: VAMP7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for VAMP7

Target: VAMP7 (Vesicle-Associated Membrane Protein 7 (VAMP7))

Alternative Name: VAMP7

Background: VAMP7 Antibody: VAMP7 is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) family, localizing to late endosomes and lysosomes. VAMP7 is thought to mediate the fusion of endosomes to their target lysosomes as well as other exocytosis events during phagocytosis and neuritogenesis. VAMP7 interacts with the VPS9 ankyrin repeat protein VARP, a protein that localizes to early endosomes and thought to regulate endosome dynamics. Together with CD82, VAMP7 can modulate the signaling of EGFR by regulating its endocytosis from the plasma membrane.

Molecular Weight: Predicted: 24, 29 kDa

Observed: 32 kDa

Gene ID: 6845

NCBI Accession: NP_001172112

UniProt: P51809