ZFYVE21 antibody (N-Term)

Catalog No. ABIN6991717

This Rabbit Polyclonal antibody specifically detects ZFYVE21 in WB, ELISA and IF. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for ZFYVE21 antibody (N-Term) (ABIN6991717)

Target: ZFYVE21 (Zinc Finger, FYVE Domain Containing 21 (ZFYVE21))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZFYVE21 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-ZFYVE21 Antibody

Binding Specificity: N-Term

Specificity: At least two isoforms of ZF21 are known to exist, this antibody will detect both isoforms.

Purification: ZF21 Antibody is affinity chromatography purified via peptide column.

Immunogen: ZF21 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human ZF21. The immunogen is located within the first 50 amino acids of ZF21.

Isotype: IgG

Application Details

Application Notes: ZF21 antibody can be used for detection of ZF21 by Western blot at 1 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: ZF21 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: ZF21 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for ZFYVE21

Target: ZFYVE21 (Zinc Finger, FYVE Domain Containing 21 (ZFYVE21))

Alternative Name: ZF21

Background: ZF21 Antibody: ZF21 was initially identified as protein that could bind to the cytoplasmic tail of MT1-MMP (Membrane-type 1 matrix metalloproteinase), a potent invasion-promoting protease. ZF21 is a member of a protein family characterized by the presence of a phosphatidylinositol 3-phosphate-binding FYVE domain and regulates focal adhesions (FAs) and cell movement. Knockdown of ZF21 expression resulted in a delay of FA disassembly following induction of synchronous disassembly of FAs by nocodazole treatment, suggesting that ZF21 is involved in FA disassembly. ZF21 contains a noncanonical pleckstrin homology domain that is a possible therapeutic target to treat metastatic cancer.

Gene ID: 79038

NCBI Accession: NP_001185882

UniProt: Q9BQ24