TOX antibody (N-Term)

Catalog No. ABIN6991735

This Rabbit Polyclonal antibody specifically detects TOX in WB, ELISA and IF. It exhibits reactivity toward Human.

Quick Overview for TOX antibody (N-Term) (ABIN6991735)

Target: TOX (Thymus High Mobility Group Box Protein (TOX))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TOX antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-TOX Antibody

Binding Specificity: AA 130-180, N-Term

Specificity: No alternatively spliced transcript variants have been observed.

Purification: TOX Antibody is affinity chromatography purified via peptide column.

Immunogen: TOX antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human TOX. The immunogen is located within amino acids 130 - 180 of TOX.

Isotype: IgG

Application Details

Application Notes: TOX antibody can be used for detection of TOX by Western blot at 1 - 2 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: TOX Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: TOX antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for TOX

Target: TOX (Thymus High Mobility Group Box Protein (TOX))

Alternative Name: TOX

Background: TOX Antibody: TOX (thymocyte selection-associated high mobility group (HMG) box protein) is a member of the HMG box family of DNA-binding proteins and likely plays a role in the regulation of T-cell development. It is a 526 amino acid nuclear protein and the expression of TOX is upregulated by pre-T cell receptor (pre-TCR) and TCR activation in immature thymocytes. TOX-mediated positive selection is associated with up-regulation of RUNX3 and is calcineurin dependent. TOX-dependent transition to the CD4+CD8 stage is required for development of class II major histocompatibility complex-specific T cells.

Molecular Weight: 58 kDa

Gene ID: 9760

NCBI Accession: NP_055544

UniProt: O94900