RGS22 antibody (N-Term)

Catalog No. ABIN6991738

This Rabbit Polyclonal antibody specifically detects RGS22 in ELISA, WB and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RGS22 antibody (N-Term) (ABIN6991738)

Target: RGS22 (Regulator of G-Protein Signaling 22 (RGS22))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RGS22 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-RGS22 Antibody

Binding Specificity: AA 870-920, N-Term

Specificity: At least four isoforms of RGS22 are known to exist, this antibody will detect the three longest isoforms. RGS22 antibody is predicted to not cross-react with other RGS proteins

Purification: RGS22 Antibody is affinity chromatography purified via peptide column.

Immunogen: RGS22 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human RGS22. The immunogen is located within amino acids 870 - 920 of RGS22.

Isotype: IgG

Application Details

Application Notes: RGS22 antibody can be used for detection of RGS22 by Western blot at 1 - 2 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: RGS22 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: RGS22 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for RGS22

Target: RGS22 (Regulator of G-Protein Signaling 22 (RGS22))

Alternative Name: RGS22

Background: RGS22 Antibody: Regulator of G-protein signaling (RGS) proteins contain an 120 amino acid conserved domain, termed the RGS domain, that acts as a GTPase-activating protein that acts to reduce the signal transmitted by the receptor-activated G-alpha subunit. RGS22 is a recently identified member of this family that localizes to the testis and can interact with guanine nucleotide binding proteins alpha 11, 12, and 13 (GNA11, GNA12, and GNA13). While RGS22 has been postulated to play a role in spermiogenesis in the testis, it is also expressed in several cancer cell lines with an epithelial origin and associated with cancer metastasis. Its overexpression in a highly metastatic cancer causes a decrease in cell migration and a reduction of the invasive potential of the cells, suggesting that RGS22 may be a potential prognostic biomarker for metastasis.

Molecular Weight: 139 kDa

Gene ID: 26166

NCBI Accession: NP_056483

UniProt: Q8NE09

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling