CBX4 antibody (N-Term)

Catalog No. ABIN6991741

This Rabbit Polyclonal antibody specifically detects CBX4 in WB, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CBX4 antibody (N-Term) (ABIN6991741)

Target: CBX4 (Chromobox Homolog 4 (CBX4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CBX4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-CBX4 Antibody

Binding Specificity: AA 50-100, N-Term

Specificity: Two alternatively spliced transcript variants have been observed.

Purification: CBX4 Antibody is affinity chromatography purified via peptide column.

Immunogen: CBX4 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human CBX4. The immunogen is located within amino acids 50 - 100 of CBX4.

Isotype: IgG

Application Details

Application Notes: CBX4 antibody can be used for detection of CBX4 by Western blot at 1 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: CBX4 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: CBX4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for CBX4

Target: CBX4 (Chromobox Homolog 4 (CBX4))

Alternative Name: CBX4

Background: CBX4 Antibody: Polycomb group (PcG) proteins are chromatin-associated proteins which are important for embryonic and adult stem cell self-renewal and maintenance. At least two distinct human PcG complexes have been identified. Polycomb repressive complex I (PRC1) contains CBX4/Pc2, HPH1 and RING domain-containing proteins (RING1, RING2 and BMI1). CBX4 (Chromobox protein homolog 4 or PC2), which functions as a transcriptional suppressor, is a candidate of KyoT2-binding proteins. It is a E3 SUMO-protein ligase which facilitates SUMO1 conjugation. Depletion of CBX4 results in decreased cellular resistance to ionizing radiation. CBX4 is directly involved in the cellular response to DNA damage.

Molecular Weight: 62 kDa

Gene ID: 8535

NCBI Accession: NP_003646

UniProt: O00257