CBX4 antibody (N-Term)

Catalog No. ABIN2779230

Cited in 1 publication. The Rabbit Polyclonal anti-CBX4 antibody (ABIN2779230) specifically detects CBX4 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit and Zebrafish (Danio rerio) samples.

Quick Overview for CBX4 antibody (N-Term) (ABIN2779230)

Target: CBX4 (Chromobox Homolog 4 (CBX4))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CBX4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CBX4 Antibody

Binding Specificity: N-Term

Purpose: CBX4 Antibody - N-terminal region

Sequence: LLIAFQNRER QEQLMGYRKR GPKPKPLVVQ VPTFARRSNV LTGLQDSSTD

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 77%

Characteristics: This is a rabbit polyclonal antibody against CBX4. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CBX4

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP30002-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-CBX4 antibody (ABIN2779230)

Hao, Midic, Garriga, Latham: "Contribution of CBX4 to cumulus oophorus cell phenotype in mice and attendant effects in cumulus cell cloned embryos." in: Physiological genomics, Vol. 46, Issue 2, pp. 66-80, (2014) (PubMed).

Target Details for CBX4

Target: CBX4 (Chromobox Homolog 4 (CBX4))

Alternative Name: CBX4

Background:

Background Information: The polycomb group (PcG) protein HPC2, which functions as a transcriptional suppressor, is a candidate of KyoT2-binding proteins. Pc2 dramatically enhances CtBP sumoylation. Pc2 is a SUMO E3, and Polycomb Group bodies may be sumoylation centers.

Gene Name: Chromobox homolog 4

Alternative Symbols: PC2, NBP16

Protein Name: E3 SUMO-protein ligase CBX4

Molecular Weight: 61kDa

Gene ID: 8535

NCBI Accession: NP_003646

UniProt: O00257