Pellino 1 antibody (C-Term)

Catalog No. ABIN6991769

The Rabbit Polyclonal anti-Pellino 1 antibody has been validated for WB, ELISA, ICC and IF. It is suitable to detect Pellino 1 in samples from Human, Mouse and Rat.

Quick Overview for Pellino 1 antibody (C-Term) (ABIN6991769)

Target: Pellino 1 (PELI1) (Pellino E3 Ubiquitin Protein Ligase 1 (PELI1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Pellino 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-Pellino 1 Antibody

Binding Specificity: AA 300-350, C-Term

Purification: Pellino 1 Antibody is affinity chromatography purified via peptide column.

Immunogen: Rabbit polyclonal Pellino 1 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human Pellino 1. The immunogen is located within amino acids 300 - 350 of Pellino 1.

Isotype: IgG

Application Details

Application Notes: Pellino 1 antibody can be used for detection of Pellino 1 by Western blot at 1 - 2 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Pellino 1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Pellino 1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for Pellino 1

Target: Pellino 1 (PELI1) (Pellino E3 Ubiquitin Protein Ligase 1 (PELI1))

Alternative Name: Pellino 1

Background: Pellino 1 Antibody: The Pellino proteins are a highly homologous family of E3 ubiquitin ligases that act as upstream mediators in Toll-like receptor (TLR) pathways that lead to activation of MAP kinases and transcription factors. Pellino 1 is required for interleukin-1-mediated signaling through its interaction with the IRAK4-IRAK-TRAF6 complex, ultimately resulting in the activation of NF-κ,B. Like other members of the Pellino family, Pellino 1 is an E3 ubiquitin ligase, able to catalyze the polyubiquitination of IRAK1. It is activated via phosphorylation by either IRAK1 and IRAK4 or the IKK-related kinases IKK-ε, and TBK1. In addition to phosphorylation, Pellino 1 activity is also modulated via ubiquitination and sumoylation.

Molecular Weight: 46 kDa

Gene ID: 57162

NCBI Accession: NP_065702

UniProt: Q96FA3

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades