LMX1B antibody (C-Term)

Catalog No. ABIN6991776

The Rabbit Polyclonal anti-LMX1B antibody (ABIN6991776) specifically detects LMX1B in WB, ELISA, IHC (p) and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for LMX1B antibody (C-Term) (ABIN6991776)

Target: LMX1B (LIM Homeobox Transcription Factor 1, beta (LMX1B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LMX1B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-LMX1B Antibody

Binding Specificity: AA 280-330, C-Term

Specificity: At least three isoforms are known to exist. This antibody is predicted to not cross-react with LMX1A.

Purification: LMX1B Antibody is affinity chromatography purified via peptide column.

Immunogen: Rabbit polyclonal LMX1B antibody was raised against a 17 amino acid peptide near the carboxy terminus of human LMX1B. The immunogen is located within amino acids 280 - 330 of LMX1B.

Isotype: IgG

Application Details

Application Notes: LMX1B antibody can be used for detection of LMX1B by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: LMX1B Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: LMX1B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for LMX1B

Target: LMX1B (LIM Homeobox Transcription Factor 1, beta (LMX1B))

Alternative Name: LMX1B

Background: LMX1B Antibody: The LIM homeobox transcription factor 1B (LMX1B) belongs to the LIM-homeodomain family. Members of this family are known to be important for pattern formation during development. LMX1B regulates mid-hindbrain patterning, LMX1B-null mice embryos have a severe reduction in the number of midbrain dopaminergic neurons compared to wild-type. While LMX1B appears to be important for both the development and the survival of dopamine neurons, the related LMX1A is crucial for the differentiation of these cells. However, LMX1A and LMX1B function cooperatively to regulated the proliferation, specification and differentiation of midbrain dopaminergic neuronal progenitors.

Molecular Weight: 45 kDa

Gene ID: 4010

NCBI Accession: NP_001167617

UniProt: O60663

Pathways: Dopaminergic Neurogenesis