WIPI2 antibody (N-Term)

Catalog No. ABIN6991783

This Rabbit Polyclonal antibody specifically detects WIPI2 in WB and ELISA. It exhibits reactivity toward Human and Rat.

Quick Overview for WIPI2 antibody (N-Term) (ABIN6991783)

Target: WIPI2 (WD Repeat Domain, Phosphoinositide Interacting 2 (WIPI2))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WIPI2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-WIPI2 Antibody

Binding Specificity: N-Term

Specificity: WIPI2 antibody is human and rat reactive. Multiple isoforms of WIPI2 are known to exist. WIPI2 antibody is predicted to not cross-react with WIPI1.

Purification: WIPI2 Antibody is affinity chromatography purified via peptide column.

Immunogen: Rabbit polyclonal WIPI2 antibody was raised against a 17 amino acid peptide near the amino terminus of human WIPI2. The immunogen is located within the first 50 amino acids of WIPI2.

Isotype: IgG

Application Details

Application Notes: WIPI2 antibody can be used for detection of WIPI2 by Western blot at 1 - 2 μ,g/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: WIPI2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: WIPI2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for WIPI2

Target: WIPI2 (WD Repeat Domain, Phosphoinositide Interacting 2 (WIPI2))

Alternative Name: WIPI2

Background: WIPI2 Antibody: WD repeat proteins play a role in many essential biologic functions, regulating the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. WIPI2, also known as ATG18B or ATG21, is a human homolog to yeast ATG18 and contains three WD repeats and has a 7-bladed propeller structure with a conserved motif that facilitates its interaction with other proteins. It is recruited to early autophagosomal structures along with Atg16L and ULK1 and is required for the formation of LC3-positive autophagosomes. Along with the highly related WIPI1, WIPI2 is found at the plasma membrane in addition to autophagosomal membranes.

Molecular Weight: 50 kDa

Gene ID: 26100

NCBI Accession: NP_056425

UniProt: Q9Y4P8