GABARAP antibody (N-Term)

Catalog No. ABIN6991865

The Rabbit Polyclonal anti-GABARAP antibody is suitable to detect GABARAP in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IF and IHC (p).

Quick Overview for GABARAP antibody (N-Term) (ABIN6991865)

Target: GABARAP (GABA(A) Receptor-Associated Protein (GABARAP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABARAP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GABARAP Antibody

Binding Specificity: N-Term

Specificity: Multiple isoforms of GABARAP exist due to alternative splicing events.

Purification: GABARAP Antibody is affinity chromatography purified via peptide column.

Immunogen: GABARAP antibody was raised against a 19 amino acid peptide near the amino terminus of human GABARAP. The immunogen is located within the first 50 amino acids of GABARAP.

Isotype: IgG

Application Details

Application Notes: GABARAP antibody can be used for detection of GABARAP by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: GABARAP Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: GABARAP antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for GABARAP

Target: GABARAP (GABA(A) Receptor-Associated Protein (GABARAP))

Alternative Name: GABARAP

Background: GABARAP Antibody: Gamma-aminobutyric acid (GABA) is the main inhibitory transmitter by increasing a Cl-conductance that inhibits neuronal firing in the central nervous system. It has been shown to activate both ionotropic (GABAA) and metabotropic (GABAB) receptors as well as a third class of receptors called GABAC. GABARAP (GABAA receptor-associated protein) links GABAA receptors to the cytoskeleton and may play a role in intracellular transport of GABAA receptors and its interaction with the cytoskeleton. GABARAP belongs to the MAP1 or ATG8 like family and recent studies show that MAPK15/ERK8 is acting through interaction with ATG8 family proteins to regulate autophagy.

Molecular Weight: Predicted: 13kDa

Observed: 15 kDa

Gene ID: 11337

NCBI Accession: NP_009209

UniProt: O95166

Pathways: Autophagy