AQP2 antibody (C-Term)

Catalog No. ABIN6991948

This Rabbit Polyclonal antibody specifically detects AQP2 in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for AQP2 antibody (C-Term) (ABIN6991948)

Target: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AQP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-AQP2 Antibody

Binding Specificity: C-Term

Specificity: AQP2 antibody is human, mouse and rat reactive. This antibody is predicted to not cross-react with other members of the aquaporin protein family.

Purification: AQP2 antibody is affinity chromatography purified via peptide column.

Immunogen: AQP2 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human AQP2. The immunogen is located within the last 50 amino acids of AQP2.

Isotype: IgG

Application Details

Application Notes: AQP2 antibody can be used for detection of AQP2 by Western blot at 1 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: AQP2 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: AQP2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for AQP2

Target: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Alternative Name: AQP2

Background: Aquaporins are membrane proteins that serve in the transfer of water and small solutes across cellular membranes. One such aquaporin, aquaporin-2 (AQP2) is located in the kidney collecting tubule and plays a critical role in water reabsorbtion (1). AQP2 is mainly localized in intracellular vesicles but upon stimulation with anti-diuretic hormone (ADH), AQP2 is translocated to the apical plasma membrane by exocytic fusion of AQP2-bearing vesicles (2). Mutations in this gene have been linked to autosomal dominant and recessive forms of nephrogenic diabetes insipidus (3).

Molecular Weight: Predicted: 30 kDa

Observed: 28 kDa

Gene ID: 359

NCBI Accession: NP_000477

UniProt: P41181

Pathways: Response to Water Deprivation