GRK6 antibody (C-Term)

Catalog No. ABIN6991952

The Rabbit Polyclonal anti-GRK6 antibody is suitable to detect GRK6 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IF and IHC (p).

Quick Overview for GRK6 antibody (C-Term) (ABIN6991952)

Target: GRK6 (G Protein-Coupled Receptor Kinase 6 (GRK6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRK6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GRK6 Antibody

Binding Specificity: C-Term

Specificity: GRK6 antibody is human, mouse and rat reactive. Multiple isoforms of GRK6 are known to exist. This antibody is predicted to not cross-react with other members of the GRK protein family.

Purification: GRK6 antibody is affinity chromatography purified via peptide column.

Immunogen: GRK6 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human GRK6. The immunogen is located within the last 50 amino acids of GRK6.

Isotype: IgG

Application Details

Application Notes: GRK6 antibody can be used for detection of GRK6 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in rat samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: GRK6 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: GRK6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for GRK6

Target: GRK6 (G Protein-Coupled Receptor Kinase 6 (GRK6))

Alternative Name: GRK6

Background: The G protein-coupled receptor kinases (GRKs) are a versatile family of kinases that play a critical role in cancer metastasis through their regulation of G-protein coupled receptors (GPCRs) involved in growth factor mediated cell migration (1,2). Phosphorylation of receptors by GRKs appears to be strictly dependent on the receptor being in its agonist-activated state (1). GRK6 is one of 7 members of the GRK serine/threonine kinase subfamily, which has been shown to modulate the Wnt signaling pathway via phosphorylation of LRP6 (3,4), and the insulin-like growth factor signaling pathway (4). GRK6 may also play a role in immune system function (5).

Molecular Weight: Predicted: 63 kDa

Observed: 63 kDa

Gene ID: 2870

NCBI Accession: NP_001004106

UniProt: P43250

Pathways: Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Negative Regulation of Transporter Activity