SUZ12 antibody (AA 400-450)

Catalog No. ABIN6991970

The Rabbit Polyclonal anti-SUZ12 antibody is suitable to detect SUZ12 in samples from Human. It has been validated for WB, ELISA, IF and IHC (p).

Quick Overview for SUZ12 antibody (AA 400-450) (ABIN6991970)

Target: SUZ12 (SUZ12 Polycomb Repressive Complex 2 Subunit (SUZ12))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUZ12 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SUZ12 Antibody

Binding Specificity: AA 400-450

Specificity: SUZ12 antibody is human specific.

Purification: SUZ12 antibody is affinity chromatography purified via peptide column.

Immunogen: SUZ12 antibody was raised against a 19 amino acid peptide near the center of human SUZ12. The immunogen is located within amino acids 400 - 450 of SUZ12.

Isotype: IgG

Application Details

Application Notes: SUZ12 antibody can be used for detection of SUZ12 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: SUZ12 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: SUZ12 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for SUZ12

Target: SUZ12 (SUZ12 Polycomb Repressive Complex 2 Subunit (SUZ12))

Alternative Name: SUZ12

Background: The Polycomb group (PcG) genes contribute to the maintenance of cell identity, cell cycle regulation and oncogenesis (1). SUZ12 (suppressor of zeste 12) is a 90 - 95 kDa member of the polycomb group of transcriptional regulators (2). It is part of a transcription repression complex termed PRC2 (Polycomb repressive complex 2) that methylates histones in the nucleus, resulting in homeotic (pattern-inducing) gene silencing. It contains one C2H2-type zinc finger region and a C-terminal VEFS-box (3,4). SUZ12 is overexpressed in breast and colon cancer. SUZ12 may be a cause of endometrial stromal tumors (5).

Molecular Weight: Predicted: 81 kDa

Observed: 96 kDa

Gene ID: 23512

NCBI Accession: NP_056170

UniProt: Q15022