TAF9 antibody (AA 110-160)

Catalog No. ABIN6991978

This Rabbit Polyclonal antibody specifically detects TAF9 in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for TAF9 antibody (AA 110-160) (ABIN6991978)

Target: TAF9 (RNA Polymerase II TBP-Associated Factor Subunit G (TAF9))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TAF9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TAF9 Antibody

Binding Specificity: AA 110-160

Specificity: TAF9 antibody is human, mouse and rat reactive. Multiple isoforms of TAF9 are known to exist. TAF9 is predicted to not cross-react with other TAF family members.

Purification: TAF9 antibody is affinity chromatography purified via peptide column.

Immunogen: TAF9 antibody was raised against a 17 amino acid peptide near the center of human TAF9. The immunogen is located within amino acids 110 - 160 of TAF9.

Isotype: IgG

Application Details

Application Notes: TAF9 antibody can be used for detection of TAF9 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry at 5 μ,g/mL. For Immunoflorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: TAF9 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: TAF9 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for TAF9

Target: TAF9 (RNA Polymerase II TBP-Associated Factor Subunit G (TAF9))

Alternative Name: TAF9

Background: Initiation of transcription by RNA polymerase II is coordinated by the transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and the TBP-associated factors (TAFs). TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation (1). TAF9 is one of the smaller subunits of TFIID that binds to the basal transcription factor GTF2B as well as to several transcriptional activators such as p53 and VP16 (2).

Molecular Weight: Predicted: 29 kDa

Observed: 28 kDa

Gene ID: 6880

NCBI Accession: NP_003178

UniProt: Q16594

Pathways: Negative Regulation of intrinsic apoptotic Signaling