TET1 antibody (C-Term)

Catalog No. ABIN6991985

The Rabbit Polyclonal anti-TET1 antibody has been validated for WB, ELISA, IF and IHC (p). It is suitable to detect TET1 in samples from Human, Mouse and Rat.

Quick Overview for TET1 antibody (C-Term) (ABIN6991985)

Target: TET1 (Tet Methylcytosine Dioxygenase 1 (TET1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TET1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TET1 Antibody

Binding Specificity: AA 2030-2080, C-Term

Specificity: TET1 antibody is human, mouse and rat reactive. This antibody is predicted to not cross-react with TET2 and TET3.

Purification: TET1 antibody is affinity chromatography purified via peptide column.

Immunogen: TET1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human TET1. The immunogen is located within amino acids 2030 - 2080 of TET1.

Isotype: IgG

Application Details

Application Notes: TET1 antibody can be used for detection of TET1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry at 5 μ,g/mL. For Immunoflorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in rat samples, Immunohistochemistry in human samples, Immunocytochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: TET1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: TET1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for TET1

Target: TET1 (Tet Methylcytosine Dioxygenase 1 (TET1))

Alternative Name: TET1

Background: TET1, a member of the ten-eleven-translocation (TET) family of genes, was initially discovered as a fusion partner of MLL in acute myeloid leukemias containing the t(10,11)(q22,q23) (1). It is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine (2). TET1 expression is regulated during inflammation in both THP-1 and primary dendritic cells, and is a negative transcriptional regulator of IL-1beta following and inflammatory stimulus (3). Recent evidence has shown that TET1 is part of a signaling pathway that regulates breast cancer growth and metastasis (4).

Molecular Weight: Predicted: 235 kDa

Observed: 240 kDa

Gene ID: 80312

NCBI Accession: NP_085128

UniProt: Q8NFU7

Pathways: Stem Cell Maintenance, Warburg Effect