TET1 antibody (C-Term)

Catalog No. ABIN6972860

Product Details anti-TET1 Antibody

Target: TET1 (Tet Methylcytosine Dioxygenase 1 (TET1))

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TET1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Characteristics: Tet1 (Ten-Eleven Translocation-1, methylcytosine dioxygenase) is a protein that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) which can lead to cytosine demethylation by either further oxidation via Tet proteins to 5-formylcytosine (5fC) and 5-carboxycytosine (5caC) or deamination into 5-hydroxymethyluracil (5hmU) and then subsequent replacement by unmethylated cytosine by the base excision repair system. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. Tet1 preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and polycomb-repressed genes. By controlling the levels of 5mC and 5hmC at gene promoters, it may regulate the gene expression silencing induced by cytosine methylation. May have a dual function by also repressing the expression of a subset of genes through recruitment of transcriptional repressors to promoters. Involved in the balance between pluripotency and lineage commitment of cells, it plays a role in embryonic stem cells maintenance and inner cell mass cell specification. Tet1 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunohistochemistry and Western blot, it has been shown to react with Human and Mouse samples.

Purification: Affinity Purified

Immunogen: This antibody was raised against a peptide within the C-terminal region of mouse Tet1.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for TET1

Target: TET1 (Tet Methylcytosine Dioxygenase 1 (TET1))

Alternative Name: Tet1 (TET1 Products)

Synonyms: CXXC6 antibody, LCX antibody, bA119F7.1 antibody, 2510010B09Rik antibody, AA517754 antibody, BB001228 antibody, Cxxc6 antibody, D10Ertd17e antibody, mKIAA1676 antibody, tet methylcytosine dioxygenase 1 antibody, solute carrier family 25 member 16 antibody, TET1 antibody, Tet1 antibody, SLC25A16 antibody

Molecular Weight: 215 kDa

NCBI Accession: NP_081660

Pathways: Stem Cell Maintenance, Warburg Effect