MIB1 antibody (C-Term)

Catalog No. ABIN6992000

The Rabbit Polyclonal anti-MIB1 antibody has been validated for ELISA, WB, ICC and IF. It is suitable to detect MIB1 in samples from Human, Mouse and Rat.

Quick Overview for MIB1 antibody (C-Term) (ABIN6992000)

Target: MIB1 (Mindbomb E3 Ubiquitin Protein Ligase 1 (MIB1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MIB1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-MIB1 Antibody

Binding Specificity: AA 920-970, C-Term

Specificity: MIB1 antibody is human, mouse and rat reactive. At least three isoforms of MIB1 are known to exist.

Purification: MIB1 antibody is affinity chromatography purified via peptide column.

Immunogen: MIB1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human MIB1. The immunogen is located within amino acids 920 - 970 of MIB1.

Isotype: IgG

Application Details

Application Notes: MIB1 antibody can be used for detection of MIB1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunocytochemistry at 5 μ,g/mL. For Immunoflorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: MIB1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: MIB1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for MIB1

Target: MIB1 (Mindbomb E3 Ubiquitin Protein Ligase 1 (MIB1))

Alternative Name: MIB1

Background: The E3 ubiquitin-protein ligase MIB1, also known as Mindbomb homolog 1, contains multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase (1). MIB1 interacts with and regulates the cellular expression of the death-associated protein kinase 1 (DAPK1) protein by promoting its ubiquitination and degradation (1). It also positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis (2).

Molecular Weight: Predicted: 89, 102, 111 kDa

Observed: 103 kDa

Gene ID: 57534

NCBI Accession: NP_065825

UniProt: Q86YT6

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection