MFN2 antibody (AA 250-300)

Catalog No. ABIN6992022

This anti-MFN2 antibody is a Rabbit Polyclonal antibody detecting MFN2 in WB, ELISA, IF and IHC (p). Suitable for Human, Rat and Mouse.

Quick Overview for MFN2 antibody (AA 250-300) (ABIN6992022)

Target: MFN2 (Mitofusin 2 (MFN2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MFN2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MFN2 Antibody

Binding Specificity: AA 250-300

Specificity: MFN2 antibody is human, mouse and rat reactive. At least three isoforms of MFN2 are known to exist. MFN2 antibody is predicted to not cross-react with MFN1.

Purification: MFN2 antibody is affinity chromatography purified via peptide column.

Immunogen: MFN2 antibody was raised against a 17 amino acid peptide near the center of human MFN2. The immunogen is located within amino acids 250 - 300 of MFN2.

Isotype: IgG

Application Details

Application Notes: MFN2 antibody can be used for detection of MFN2 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry at 5 μ,g/mL. For Immunoflorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: MFN2 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: MFN2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for MFN2

Target: MFN2 (Mitofusin 2 (MFN2))

Alternative Name: MFN2

Background: Mitofusin 2 (MFN2) and the related protein MFN1 are mitochondrial membrane GTPase proteins that play a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes (1,2). MFN2 is ubiquitously expressed, and found in both the ER and outer mitochondrial membrane. MFN2 has two key domains: a coiled coil region that mediates MFN2 binding and a GTPase domain that likely plays a role in fusion (3,4). Both domains are essential for embryonic development and may play a role in the pathobiology of obesity. Overexpression of MFN2 causes mitochondrial dysfunction and cell death (5).

Molecular Weight: Predicted: 83 kDa

Observed: 90 kDa

Gene ID: 9927

NCBI Accession: NP_055689

UniProt: O95140

Pathways: Skeletal Muscle Fiber Development