PDCL3 antibody (C-Term)

Catalog No. ABIN6992031

This anti-PDCL3 antibody is a Rabbit Polyclonal antibody detecting PDCL3 in WB, ELISA, IF and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for PDCL3 antibody (C-Term) (ABIN6992031)

Target: PDCL3 (Phosducin-Like 3 (PDCL3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDCL3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PDCL3 Antibody

Binding Specificity: C-Term

Specificity: PDCL3 antibody is human, mouse and rat reactive. PDCL3 antibody is predicted to not cross-react with other members of the PDCL protein family.

Purification: PDCL3 antibody is affinity chromatography purified via peptide column.

Immunogen: PDCL3 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human PDCL3. The immunogen is located within the last 50 amino acids of PDCL3.

Isotype: IgG

Application Details

Application Notes: PDCL3 antibody can be used for detection of PDCL by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry at 5 μ,g/mL. For Immunoflorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PDCL3 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: PDCL3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for PDCL3

Target: PDCL3 (Phosducin-Like 3 (PDCL3))

Alternative Name: PDCL3

Background: Phosducin-like proteins (PhLPs) are a conserved family of proteins with thioredoxin-like domains that were initially identified as modulators of G protein signaling (1,2). PDCL3 is highly homologous to PDCL and shares an N-terminal helix domain and a C-terminal thioredoxin-fold (Trx-fold) domain (3). Along with the related protein PDCL2, PDCL3 interacts with the chaperonin CCT and modulates CCT-mediated actin and tubulin folding (4). Modulation of PDCL3 levels by MAPK phosphorylation and RhoA-dependent changes also promote cytoskeletal remodeling (5).

Molecular Weight: Predicted: 26 kDa

Observed: 25 kDa

Gene ID: 79031

NCBI Accession: NP_076970

UniProt: Q9H2J4