CYBB antibody (N-Term)

Catalog No. ABIN6992043

This anti-CYBB antibody is a Rabbit Polyclonal antibody detecting CYBB in WB, ELISA, IF and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for CYBB antibody (N-Term) (ABIN6992043)

Target: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYBB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CYBB Antibody

Binding Specificity: AA 140-190, N-Term

Specificity: NOX2 antibody is human, mouse and rat reactive. At least two isoforms are known to exist, this antibody will detect both isoforms. NOX2 is predicted to not cross-react with other NOX proteins.

Purification: NOX2 antibody is affinity chromatography purified via peptide column.

Immunogen: Rabbit polyclonal NOX2 antibody was raised against a 15 amino acid peptide near the amino terminus of human NOX2. The immunogen is located within amino acids 140 - 190 of NOX2.

Isotype: IgG

Application Details

Application Notes: NOX2 antibody can be used for detection of NOX2 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 2 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in rat samples, Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: NOX2 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: NOX2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for CYBB

Target: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Alternative Name: NOX2

Background: The NOX family of NAPDH oxidases is comprised of seven transmembrane proteins that oxidize intracellular NAPDH/NADH, causing electron transport across the membrane and the reduction of molecular oxygen to superoxide (1). NOX2, also known as cytochrome b beta (CYBB) is one of two proteins that make up Cytochrome b-245, thought to be a primary component of the microbicidal oxidase system of phagocytes. NOX2 deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD) (2). Activation of the NOX2 enzyme complex in microglia is thought to be neurotoxic and may play a role in Alzheimer's and Parkinson's disease (3).

Molecular Weight: Predicted: 59, 63 kDa

Observed: 58 kDa

Gene ID: 1536

NCBI Accession: NP_000388

UniProt: P04839