NADPH Oxidase 4 antibody (N-Term)

Catalog No. ABIN6992045

Product Details anti-NADPH Oxidase 4 Antibody

Target: NADPH Oxidase 4 (NOX4)

Binding Specificity: AA 70-120, N-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NADPH Oxidase 4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: NOX4 antibody is human, mouse, and rat reactive. At least four isoforms of NOX4 are known to exist. NOX4 is predicted to not cross-react with other NOX proteins.

Purification: NOX4 antibody is affinity chromatography purified via peptide column.

Immunogen: NOX4 antibody was raised against a 14 amino acid peptide near the amino terminus of human NOX4. The immunogen is located within amino acids 70 - 120 of NOX4.

Isotype: IgG

Application Details

Application Notes: NOX4 antibody can be used for detection of NOX4 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: NOX4 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: NOX4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for NADPH Oxidase 4

Target: NADPH Oxidase 4 (NOX4)

Alternative Name: NOX4 (NOX4 Products)

Background: The NOX family of NAPDH oxidases is comprised of seven transmembrane proteins that oxidize intracellular NAPDH/NADH, causing electron transport across the membrane and the reduction of molecular oxygen to superoxide (1). NOX4 is is expressed in multiple tissues and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS) (2,3). Unlike other NOX proteins, NOX4 does not require cytosolic subunits and thus is constitutively active (4). The function of NOX4 remains unclear as it plays both protective and deleterious roles in cellular metabolism.

Molecular Weight: Predicted: 64 kDa

Observed: 68 kDa

Gene ID: 50507

NCBI Accession: NP_058627

UniProt: Q9NPH5

Pathways: Carbohydrate Homeostasis, Smooth Muscle Cell Migration