CD55 antibody (C-Term)

Catalog No. ABIN6992050

This Rabbit Polyclonal antibody specifically detects CD55 in WB, IF, ELISA and IHC (p). It exhibits reactivity toward Human.

Quick Overview for CD55 antibody (C-Term) (ABIN6992050)

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD55 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CD55 Antibody

Binding Specificity: AA 360-410, C-Term

Specificity: DAF antibody is human specific. At least two isoforms of DAF are known to exist, this antibody will only detect the longest isoform.

Purification: DAF antibody is affinity chromatography purified via peptide column.

Immunogen: DAF antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DAF. The immunogen is located within amino acids 360 - 410 of DAF.

Isotype: IgG

Application Details

Application Notes: DAF body can be used for detection of DAF by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: DAF antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: DAF antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for CD55

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Alternative Name: DAF

Background: The decay-accelerating factor (DAF), also known as CD55, is an integral membrane glycoprotein that is involved in the regulation of the complement cascade (1). DAF limits excessive complement activation by accelerating their decay following DAF binding, thereby disrupting the cascade and preventing damage to host cells (2). Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM) (3).

Molecular Weight: Predicted: 48 kDa

Observed: 49 kDa

Gene ID: 1604

NCBI Accession: NP_001108224

UniProt: P08174

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity