WWC1 antibody (N-Term)

Catalog No. ABIN6992072

The Rabbit Polyclonal anti-WWC1 antibody has been validated for WB, ELISA, IF and IHC (p). It is suitable to detect WWC1 in samples from Human, Mouse and Rat.

Quick Overview for WWC1 antibody (N-Term) (ABIN6992072)

Target: WWC1 (WW and C2 Domain Containing 1 (WWC1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WWC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-WWC1 Antibody

Binding Specificity: AA 60-110, N-Term

Specificity: WWC1 antibody is human, mouse and rat reactive. At least three isoforms of WWC1 are known to exist, this antibody will detect all three isoforms.

Purification: WWC1 antibody is affinity chromatography purified via peptide column.

Immunogen: WWC1 antibody was raised against an 18 amino acid peptide near the amino terminus of human WWC1. The immunogen is located within amino acids 60 - 110 of WWC1.

Isotype: IgG

Application Details

Application Notes: WWC1 antibody can be used for detection of WWC1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: WWC1 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: WWC1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for WWC1

Target: WWC1 (WW and C2 Domain Containing 1 (WWC1))

Alternative Name: WWC1

Background: The WW and C2 domain containing 1 (WWC1) protein, also known as KIBRA, possesses two WW domains and an internal C2-like domain (1). WWC1 was originally identified as a memory performance-associated protein in humans (2) and has recently been shown to be a novel regulator of the Hippo pathway (3). WWC1 is phosphorylated by the mitotic kinases Aurora-A and -B (4), and in turn activates the Aurora kinases and is required for precise chromosome alignment during mitosis (5).

Molecular Weight: Predicted: 123 kDa

Observed: 110 kDa

Gene ID: 23286

NCBI Accession: NP_001155133

UniProt: Q8IX03