Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

HES5 antibody (N-Term)

The Rabbit Polyclonal anti-HES5 antibody has been validated for WB, ELISA, IHC (p) and IF. It is suitable to detect HES5 in samples from Human, Mouse and Rat.
Catalog No. ABIN6992090

Quick Overview for HES5 antibody (N-Term) (ABIN6992090)

Target

See all HES5 Antibodies
HES5 (Hairy and Enhancer of Split 5 (HES5))

Reactivity

  • 29
  • 26
  • 23
  • 3
  • 2
  • 2
  • 1
Human, Mouse, Rat

Host

  • 36
  • 2
Rabbit

Clonality

  • 34
  • 4
Polyclonal

Conjugate

  • 15
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This HES5 antibody is un-conjugated

Application

  • 28
  • 13
  • 13
  • 13
  • 6
  • 4
  • 4
  • 1
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
  • Binding Specificity

    • 15
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term

    Specificity

    HES5 antibody is human, mouse and rat reactive. At least two isoforms of HES5 are known to exist, this antibody will detect both isoforms.

    Purification

    HES5 antibody is affinity chromatography purified via peptide column.

    Immunogen

    HES5 antibody was raised against a 19 amino acid peptide near the amino terminus of human HES5. The immunogen is located within the first 50 amino acids of HES5.

    Isotype

    IgG
  • Application Notes

    HES5 antibody can be used for detection of HES5 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

    Antibody validated: Western Blot in human samples, Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    HES5 antibody is supplied in PBS containing 0.02 % sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C,4 °C

    Storage Comment

    HES5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
  • Target

    HES5 (Hairy and Enhancer of Split 5 (HES5))

    Alternative Name

    HES5

    Background

    HES5 is a member of the HES family of transcriptional repressors, which are the mammalian homologs of the Drosophila Hairy and Enhancer of Split and contain basic Helix-loop-helix (bHLH) and Orange domains (1). The HES5 gene is a downstream target of the Notch signaling pathway and its expression is downregulated during human cartilage differentiation (2). HES5 can directly repress transcription of the E3 ligase FBW7-beta, creating a feedback loop that modulates Notch-mediated intestinal and neural stem cell fate decisions (3).

    Molecular Weight

    Predicted: 18 kDa

    Observed: 17 kDa

    Gene ID

    388585

    NCBI Accession

    NP_001010926

    UniProt

    Q5TA89

    Pathways

    Stem Cell Maintenance, Tube Formation
You are here:
Chat with us!