ATG4A antibody (C-Term)

Catalog No. ABIN6992095

This Rabbit Polyclonal antibody specifically detects ATG4A in WB, ELISA and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ATG4A antibody (C-Term) (ABIN6992095)

Target: ATG4A (Autophagy related 4A Cysteine Peptidase (ATG4A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG4A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ATG4A Antibody

Binding Specificity: C-Term

Specificity: ATG4A antibody is human, mouse and rat reactive. At least two isoforms of ATG4A are known to exist, this antibody will detect both isoforms. ATG4A is predicted to not cross-react with other ATG4 proteins.

Purification: ATG4A antibody is affinity chromatography purified via peptide column.

Immunogen: ATG4A antibody was raised against an 18 amino acid peptide near the carboxy terminus of human ATG4A. The immunogen is located within the last 50 amino acids of ATG4A.

Isotype: IgG

Application Details

Application Notes: ATG4A antibody can be used for detection of ATG4A by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL.

Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: ATG4A antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: ATG4A antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for ATG4A

Target: ATG4A (Autophagy related 4A Cysteine Peptidase (ATG4A))

Alternative Name: ATG4A

Background: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). ATG4A, also known as AUTL2, is one of four mammalian orthologs of the yeast ATG4 protein, all four are cysteine proteases (3). ATG4 is required for ATG8 conjugation to phosphatidylethanolamine on autophagosomal membranes. In mammals, each ATG4 homolog shows a selective preference for the ATG8 homologs (4).

Molecular Weight: Predicted: 48 kDa

Observed: 44 kDa

Gene ID: 115201

NCBI Accession: NP_443168

UniProt: Q8WYN0

Pathways: Autophagy