MYH8 antibody (N-Term)

Catalog No. ABIN6992099

Product Details anti-MYH8 Antibody

Target: MYH8 (Myosin Heavy Chain 8, Skeletal Muscle, Perinatal (MYH8))

Binding Specificity: AA 60-110, N-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYH8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: MYH8 antibody is human, mouse and rat reactive. MYH8 antibody is predicted to not cross-react with other members of the myosin heavy chain family.

Purification: MYH8 antibody is affinity chromatography purified via peptide column.

Immunogen: MYH8 antibody was raised against a 19 amino acid peptide near the amino terminus of human MYH8. The immunogen is located within amino acids 60 - 110 of MYH8.

Isotype: IgG

Application Details

Application Notes: MYH8 antibody can be used for detection of MYH8 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: MYH8 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: MYH8 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for MYH8

Target: MYH8 (Myosin Heavy Chain 8, Skeletal Muscle, Perinatal (MYH8))

Alternative Name: MYH8 (MYH8 Products)

Synonyms: MyHC-peri antibody, MyHC-pn antibody, gtMHC-F antibody, 4832426G23Rik antibody, AI327267 antibody, MHCp antibody, Myhs-p antibody, Myhsp antibody, myosin heavy chain 8 antibody, myosin, heavy polypeptide 8, skeletal muscle, perinatal antibody, MYH8 antibody, Myh8 antibody

Background: Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells (1). MYH8 (myosin, heavy chain 8, skeletal muscle, perinatal) is a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction (2,3). This gene is predominantly expressed in fetal skeletal muscle. MYH8 is regulated by phosphorylation via myosin light chain kinase (MLCK) and by intracellular Ca2+ concentrations (3). A mutation in this gene results in trismus-pseudocamptodactyly syndrome (4).

Molecular Weight: Predicted: 213 kDa

Observed: 220 kDa

Gene ID: 4626

NCBI Accession: NP_002463

UniProt: P13535