MAGEA4 antibody (N-Term)

Catalog No. ABIN6992141

The Rabbit Polyclonal anti-MAGEA4 antibody has been validated for WB, ELISA, IHC (p) and IF. It is suitable to detect MAGEA4 in samples from Human, Rat and Mouse.

Quick Overview for MAGEA4 antibody (N-Term) (ABIN6992141)

Target: MAGEA4 (Melanoma Antigen Family A, 4 (MAGEA4))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAGEA4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-MAGEA4 Antibody

Binding Specificity: N-Term

Specificity: MAGEA4 antibody is human specific. At least four isoforms of MAGEA4 are known to exist.

Purification: MAGEA4 antibody is affinity chromatography purified via peptide column.

Immunogen: MAGEA4 antibody was raised against a 16 amino acid peptide near the amino terminus of human MAGEA4. The immunogen is located within the first 50 amino acids of MAGEA4.

Isotype: IgG

Application Details

Application Notes: MAGEA4 antibody can be used for detection of MAGEA4 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: MAGEA4 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: MAGEA4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for MAGEA4

Target: MAGEA4 (Melanoma Antigen Family A, 4 (MAGEA4))

Alternative Name: MAGEA4

Background: MAGEA4 is a member of the melanoma-associated antigen family that consists of a number of antigens recognized by cytotoxic T lymphocytes (1). MAGEA4 may serve as a target for antitumoral vaccination and may play a role in embryonal development and tumor transformation or aspects of tumor progression. It is expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma and have been implicated in some hereditary disorders, such as dyskeratosis congenital (2-4).

Molecular Weight: Predicted: 35 kDa

Observed: 45 kDa

Gene ID: 4103

NCBI Accession: NP_001011550

UniProt: P43358