DEPDC1B antibody (C-Term)

Catalog No. ABIN6992160

Product Details anti-DEPDC1B Antibody

Target: DEPDC1B (DEP Domain Containing 1B (DEPDC1B))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DEPDC1B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: DEPDC1B antibody is human specific. At least two isoforms are known to exist, this antibody will only detect the largest isoform. DEPDC1B antibody is predicted to not cross-react with DEPDC1A.

Purification: DEPDC1B antibody is affinity chromatography purified via peptide column.

Immunogen: DEPDC1B antibody was raised against a 19 amino acid peptide near the carboxy terminus of human DEPDC1B. The immunogen is located within the last 50 amino acids of DEPDC1B.

Isotype: IgG

Application Details

Application Notes: DEPDC1B antibody can be used for detection of DEPDC1B by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: DEPDC1B antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: DEPDC1B antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for DEPDC1B

Target: DEPDC1B (DEP Domain Containing 1B (DEPDC1B))

Alternative Name: DEPDC1B (DEPDC1B Products)

Background: The DEP domain-containing 1B (DEPDC1B) protein was initially identified as a radiation response biomarker in human lymphoblastoid cell lines (1). Like the related protein DEPDC1A, expression of DEPDC1B correlates with a poor prognosis in cancer patients (2,3). Studies have shown that DEPDC1B levels are upregulated in non-small cell lung cancer (NSCLC) and have an inverse correlation with patient survival. Ectopic expression of DEPDC1B in NSCLC activated Wnt/B-catenin signaling and enhanced cell migration and invasion while silencing DEPDC1B expression suppressed these traits (2).

Molecular Weight: Predicted: 58 kDa

Observed: 57 kDa

Gene ID: 55789

NCBI Accession: NP_060839

UniProt: Q8WUY9