RNF121 antibody (C-Term)

Catalog No. ABIN6992161

This Rabbit Polyclonal antibody specifically detects RNF121 in WB, ELISA and ICC. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for RNF121 antibody (C-Term) (ABIN6992161)

Target: RNF121 (Ring Finger Protein 121 (RNF121))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF121 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Product Details anti-RNF121 Antibody

Binding Specificity: AA 260-310, C-Term

Specificity: RNF121 antibody is human, mouse and rat reactive. Multiple isoforms of RNF121 are known to exist.

Purification: RNF121 antibody is affinity chromatography purified via peptide column.

Immunogen: RNF121 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human RNF121. The immunogen is located within amino acids 260 - 310 of RNF121.

Isotype: IgG

Application Details

Application Notes: RNF121 antibody can be used for detection of RNF121 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry at 10 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: RNF121 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: RNF121 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for RNF121

Target: RNF121 (Ring Finger Protein 121 (RNF121))

Alternative Name: RNF121

Background: RNF121 is an evolutionarily conserved E3 ligase ring finger protein that is expressed in the endoplasmic reticulum (1). It acts downstream of the unfolded protein response regulator protein kinase-like endoplasmic reticulum kinase (PERK). Overexpression of RNF121 during the early stages of gonad development led to aberrations in germline development and gonad migration, suggesting that it plays a role in endoplasmic reticulum-associated degradation machinery (1).

Molecular Weight: Predicted: 24, 27, 36 kDa

Observed: 28 kDa

Gene ID: 552978

NCBI Accession: NP_060790

UniProt: Q9H920

Pathways: ER-Nucleus Signaling