CD276 antibody (C-Term)

Catalog No. ABIN6992208

This anti-CD276 antibody is a Rabbit Polyclonal antibody detecting CD276 in WB, ELISA, IHC (p) and IF. Suitable for Human, Mouse and Rat.

Quick Overview for CD276 antibody (C-Term) (ABIN6992208)

Target: CD276

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD276 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-CD276 Antibody

Binding Specificity: AA 484-534, C-Term

Specificity: At least three antibodies are known to exist, this antibody will detect all three isoforms.

Purification: CD276 Antibody is affinity chromatography purified via peptide column.

Immunogen: CD276 antibody was raised against a peptide corresponding to 19 amino acids near the carboxy terminus of CD276. The immunogen is located within amino acids 484 - 534 of CD276.

Isotype: IgG

Application Details

Application Notes: CD276 antibody can be used for Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for Immunohistochemistry at 2 μ,g/mL. For Immunoflorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human and mouse samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: CD276 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: CD276 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for CD276

Target: CD276

Alternative Name: CD276

Background: CD276, also known as B7-H3, was initially identified as a member of the B7 family of proteins through its homology with previously identified B7 Molecules (1). CD276 mRNA is widely expressed, but its protein expression is usually rather low (2). CD276 has been shown to play a role in both the costimulation as well as the coinhibition of T cell response (3). In a similar fashion, CD276 plays a critical role in the control of antitumor immune responses in some cases, while in others appears to mediate antitumor immunity (4). It thus joins other immune checkpoint proteins as a possible therapeutic target for at least a subset of cancers.

Molecular Weight: Predicted: 35, 43, 57 kDa

Observed: 59 kDa

Gene ID: 80381

NCBI Accession: NP_001019907

UniProt: Q5ZPR3

Pathways: Cancer Immune Checkpoints