CTLA4 antibody (Extracellular Domain)

Catalog No. ABIN6992223

This anti-CTLA4 antibody is a Mouse Monoclonal antibody detecting CTLA4 in WB, ELISA, ICC, IF and IHC (p). Suitable for Human.

Quick Overview for CTLA4 antibody (Extracellular Domain) (ABIN6992223)

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CTLA4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 1E6

Product Details anti-CTLA4 Antibody

Binding Specificity: Extracellular Domain

Purification: CTLA-4 Antibody is supplied as protein A purified IgG1.

Immunogen: CTLA-4 antibody was raised against the extracellular domain of human CTLA-4.

Isotype: IgG1

Application Details

Application Notes: CTLA-4 antibody can be used for detection of CTLA-4 by Western blot at 0.125 - 0.25 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: CTLA-4 Antibody is supplied in PBS containing 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: CTLA-4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for CTLA4

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Alternative Name: CTLA4

Background: The cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), also known as CD152, is a member of the immunoglobulin superfamily that is expressed by activated T cells and transmits an inhibitory signal to T cells (1,2). Both it and the homologous T-cell co-stimulatory protein CD28 bind to CD80 (B7-H1) and CD86 (B7-H2) on antigen-presenting cells (APCs) (3). Mutations in the CTLA-4 gene have been implicated in multiple autoimmune diseases (4). CTLA-4 also functions as an immune checkpoint protein, and anti- CTLA-4 antibodies have been successfully used in the treatment of cancer (5).

Molecular Weight: Predicted: 25 kDa

Observed: 32 kDa

Gene ID: 1493

NCBI Accession: NP_005205

UniProt: P16410

Pathways: Cancer Immune Checkpoints