CD276 antibody (Extracellular Domain)

Catalog No. ABIN6992267

The Mouse Monoclonal anti-CD276 antibody has been validated for WB, ELISA, FACS, IHC (p), ICC and IF. It is suitable to detect CD276 in samples from Human.

Quick Overview for CD276 antibody (Extracellular Domain) (ABIN6992267)

Target: CD276

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD276 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: 2H5

Product Details anti-CD276 Antibody

Binding Specificity: Extracellular Domain

Purification: B7-H3 Antibody is supplied as protein A purified IgG1.

Immunogen: B7-H3 antibody was raised against the extracellular domain of human B7-H3

Isotype: IgG1 kappa

Application Details

Application Notes: B7-H3 antibody can be used for detection of B7-H3 by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2 μ,g/mL and Immunocytochemistry starting at 1 μ,g/mL. For immunofluorescence start at 10 μ,g/mL. Flow cytometry at 1 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples, Immunocytochemistry in human samples, Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: B7-H3 Antibody is supplied in PBS containing 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: B7-H3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for CD276

Target: CD276

Alternative Name: B7H3

Background: CD276, also known as B7-H3, was initially identified as a member of the B7 family of proteins through its homology with previously identified B7 Molecules (1). CD276 mRNA is widely expressed, but its protein expression is usually rather low (2). CD276 has been shown to play a role in both the costimulation as well as the coinhibition of T cell response (3). In a similar fashion, CD276 plays a critical role in the control of antitumor immune responses in some cases, while in others appears to mediate antitumor immunity (4). It thus joins other immune checkpoint proteins as a possible therapeutic target for at least a subset of cancers.

Molecular Weight: Predicted: 57 kDa

Observed: 100 kDa

Gene ID: 80381

NCBI Accession: NP_001019907

UniProt: Q5ZPR3

Pathways: Cancer Immune Checkpoints