SARS-CoV-2 ORF3b antibody

Catalog No. ABIN6992303

This Rabbit Polyclonal antibody specifically detects SARS-CoV-2 ORF3b in ELISA, WB and IHC. It exhibits reactivity toward SARS Coronavirus-2 (SARS-CoV-2).

Quick Overview for SARS-CoV-2 ORF3b antibody (ABIN6992303)

Target: SARS-CoV-2 ORF3b

Reactivity: SARS Coronavirus-2 (SARS-CoV-2)

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Product Details

Specificity: ORF3b Antibody is predicted to not cross-react with other coronavirus family members.

Purification: SARS-CoV-2 (COVID-19) ORF3b Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-SARS-CoV-2 (COVID-19) ORF3b antibody was raised against a peptide corresponding to 16 amino acids near the central domain of SARS-CoV-2 (COVID-19) ORF3b protein. The immunogen is located within 30-80 amino acids of the SARS-CoV-2 (COVID-19) ORF3b protein.

Isotype: IgG

Application Details

Application Notes: WB: 0.25 μ,g/mL, IHC: 0.5 μ,g/mL Antibody validated: Immunohistochemistry in human samples. SARS-CoV-2 (COVID-19) ORF3b antibody can detect 2 ng of free peptide at 1 μ,g/mL in ELISA. It can detect SARS-CoV-2 ORF3b recombinant protein by ELISA and WB. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: SARS-CoV-2 (COVID-19) ORF3b antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: SARS-CoV-2 (COVID-19) ORF3b antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details

Target: SARS-CoV-2 ORF3b

Target Type: Viral Protein

Background: Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019-20 coronavirus outbreak (2). SARS-CoV-2 virus proteins include structural proteins, non-structural proteins and accessory factors. The structure of SARS-CoV-2 consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. SARS-CoV-2 non-structural protein is ORF1ab that consists of 16 proteins (nsp1-nsp16), while accessory factors include ORF3a, ORF3b, ORF6, ORF7a, ORF7b, ORF3b, ORF9b, ORF9c and ORF10. ORF3b may play a role in interferon antiviral system evasion (3).

Molecular Weight: 17kD kDa

Gene ID: 43740569

UniProt: P0DTC5, P0DTC3