GAPDH antibody (AA 2-148)

Catalog No. ABIN7428953

The Mouse Monoclonal anti-GAPDH antibody has been validated for WB, IHC and IF. It is suitable to detect GAPDH in samples from Human.

Quick Overview for GAPDH antibody (AA 2-148) (ABIN7428953)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: D1

Product Details anti-GAPDH Antibody

Binding Specificity: AA 2-148

Purpose: Anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Monoclonal Antibody

Specificity: The antibody is a mouse monoclonal antibody raised against GAPDH. It has been selected for its ability to recognize GAPDH in immunohistochemical staining and western blotting.

Cross-Reactivity: Cow, Goat, Guinea Pig, Mouse, Rat, Sheep

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) corresdonding to Gly2~Ser148 with N-terminal His Tag

Application Details

Application Notes: Western blotting: 0.1 ng/mL-100 ng/mL,Immunohistochemistry: 5-20 μg/mL,Immunofluorescence:5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background: G3PD, GAPD, Peptidyl-cysteine S-nitrosylase GAPDH

UniProt: P04406